Litesizer 500

Model : Litesizer 500

Country of Origin: USA

Particle characterization from the nano- to the micrometer range
Particle size measurements via dynamic light scattering at three different measurement angles
Determination of zeta potential using patented cmPALS
Molecular mass and refractive index measurements The Litesizer series is the perfect choice to characterize nano- and microparticles at the touch of a button. Particles in dispersion as well as peptides or macromolecules in solution can be determined over a broad concentration range, limiting the time for sample preparation. Litesizer 500 includes three measurement angles for particle size determination as well as patented cmPALS technology, which enables zeta potential measurements at low voltages with the highest repeatability and accuracy

Key Features

- Measurement principle Dynamic Light Scattering (DLS)
- Measuring range 0.3 nm to 10 μm (particle diameter)
- Measurement angles 15°, 90°, 175° 175°
- Min. concentration 0.1 mg/mL (lysozyme)
- Max. concentration 50 %w/v (sample-dependent)
- Min. sample volume 12 µL
- Accuracy Better than +/-2 % on NIST traceable standards
- Repeatability Better than +/-2 % on NIST traceable standards

Zeta potential specifications

- Measuring principle Electrophoretic Light Scattering (ELS) / cmPALS –
- Measuring range >= +/-1000 mV –
- Mobility range 10 -11 m²/V.s to 2 x 10 -7 m²/V.s –
- Size range 3.8 nm to 100 µm (diameter) –
- Min. sample concentration 0.1 mg/mL (lysozyme) –
- Max. sample concentration 70 %w/v (sample-dependent) –
- Max. sample conductivity 200 mS/cm –
- Min. sample volume 50 µL (sample-viscosity-dependent) –
- Accuracy Better than +/-10 % –
- Repeatability +/-3 % –

Molecular mass specifications

- Measuring principle Static Light Scattering (SLS) –
- Measuring range (mass) 980 Da to 20 MDa –
- Measuring range (particle size) up to 40 nm (diameter) –
- Measurement angle 90° –
- Min. sample concentration 0.1 mg/mL (lysozyme) –
- Accuracy +/-10 % –
- Repeatability +/-5 % –

Transmittance specifications

- Measuring range 0 % to 100 %
- Min. sample volume 15 µL
- Accuracy Better than +/-1 %

Refractive index specifications

- Measuring range 1.28 to 1.50 –
- Min. sample volume 1 mL –
- Accuracy +/-0.5 % –

General specifications

Light source Semiconductor laser diode / 40 mW, 658 nm
Laser warm-up time 6 min
Temperature control range 0 °C to 90 °C
Ambient operating temperature 10 °C to 35 °C